Dengue DIII Env (Subtype 4)

Dengue virus is an arthropod-borne flavivirus virus. Four antigenically distinct serotypes (1–4), cause a wide range of diseases ranging from mild dengue fever to severe dengue hemorrhagic fever and dengue shock syndrome.  It has been estimated that more than 2.5 billion people in over 100 countries are at risk of dengue infection, with several hundred thousand cases of life threatening infections occurring every year.

The flavivirus genome encodes three structural proteins: the capsid protein, premembrane protein and envelope protein; and seven non-structural proteins: NS1, NS2A, NS2B, NS3, NS4A, NS4B and NS5.  The envelope protein comprises three regions: Domain I, Domain II and Domain III. Domain I is the central domain, Domain II is the dimerization domain, while Domain III (DIII) is an immunoglobulin-like domain.  Experimental evidence has shown that DIII protein is a receptor recognition and binding domain which has been demonstrated to be highly immunogenic and is able to elicit the production of neutralizing antibodies against wild-type virus.

For this reason, DIII protein is an important immunogen for the development of a possible protein subunit vaccine and also a potential diagnostic reagent for improved clinical diagnosis of flaviviral infections.

Caution: This product is intended for research or manufacturing use only.

Product Details:

Product # BA22-C
Size 1mg
Appearance white powder
Purity >95%
Mol Weight approx 11.2kDa
Concentration 1.0 mg/ml when reconstituted as recommended
Buffer 20mM Carbonate Buffer pH 9,6
Activity Binds Dengue positive serum antibodies
Shipping Shipped at ambient temperature
Storage following reconstitution store at 4 C, else aliquot and store at -20C
Expression System E. Coli
Assay Method(s) SDS-PAGE (purity),
Immunoblot (reactivity sera)
Application Suitable for use in ELISA, Western Blot
& lateral flow assays. Each laboratory
should determine optimum working titre
for use in it’s particular application
Expiry Date As indicated
Production Date As indicated
Assay Date As indicated
Lot # As indicated