Typhoid Hemolysin E (HlyE)

Typhoid fever is an acute systemic illness caused by Salmonella enterica serovar Typhi. Typhoid fever remains a public health concern, with an estimated 22 million cases and 200,000 related deaths occurring worldwide each year.

The S. Typhi HlyE protein belongs to a class of pore-forming toxins with hemolytic and cytotoxic activities that are absent in other Salmonella enterica serovars. The HlyE protein is expressed as a monomer, but it has a tendency to form non-functional soluble multimers in aqueous solution. Serological studies have shown that S. Typhi and S. Paratyphi A strains produce substantial amounts of HlyE during human infection.

S. Typhi HlyE has been found in the plasma of patients with S. Typhi bacteremia and recent studies have identified that HlyE is an antigen that is capable of discriminating between typhoid patients and control subjects. The reactivity of different Ig isotypes (IgG, IgA and IgM) against rHlyE has also been demonstrated by ELISA. (Ong et al. Microbial Immunol 2015; 59:43-47, Chin et al DOI: 10.7860/JCDR/2016/17801.7909).

Product Details:

Product # BA13-C
Size 0,5 mg
Appearance solid white powder
Purity >95%
Mol Weight approx 34 kDa
Concentration 0.5 mg/mL when reconstituted as recommended
Buffer 20mM Na-carbonate Buffer pH10.0
Activity Binds Typhoid antibodies
Shipping Shipped at ambient temperature
Storage 15-25 C lyophilized;
4 C when reconstituted
Expression System E. Coli
Assay Method(s) SDS-PAGE (purity)
Application Suitable for use in LFIA, ELISA and Western Blot.  Each laboratory should determine optimum working titre for use in it’s particular application
Expiry Date As indicated
Production Date As indicated
Assay Date As indicated
Lot # As indicated