HIV-1 p24
(Subtype C Clone)

The double-stranded RNA genome of mature HIV-1 virions is contained within a rigid capsid structure, the major component of which is a 24 kDa protein, p24. p24 is produced from a polyprotein precursor protein (Pr55Gag), which is cleaved into the mature Gag proteins – matrix (MA/p17), capsid (CA/p24), nucleocapsid (NC/p7) and p6 – following proteolytic cleavage by the viral protease. p24 monomers assemble into conical capsids at the host cell membrane, encapsidating the p7/6-associated viral genomic RNAs before budding from the infected cell as part of mature, enveloped, infectious virions.

Following HIV infection, the markers to identify infection in their order of appearance in serum are: viral RNA, p24 antigen, and anti-HIV antibody. About 2 weeks after infection, viremia is thought to increase exponentially and then decline to a steadystate level as the humoral and cell-mediated immune responses control HIV replication. Assays (made with recombinant gp41 & gp36), can detect antibody against HIV-1 gp41 or HIV-2 gp36 in most individuals at about 3 to 4 weeks post-infection. The inclusion of p24 to detect anti-p24 antibodies can reduce this window period to 2 weeks post infection.

4-12% Denaturing (SDS)
Bis-Tris Polyacrylamide Gel

biosheet3

Caution: This product is intended for research or manufacturing use only.

Product Details:

Product # BA04B-C
Size 1mg
Appearance solid white powder
Purity >95%
Mol Weight 26.7kDa (apparent mobility on
12% bis/tri polyacrylamide gel
Concentration 1.0 mg/ml when reconstituted
as recommended
Buffer Phospahte Buffered Saline (PBS), pH7.4
Activity Binds HIV-1 positive serum antibodies
Shipping Shipped at ambient temperature
Storage 15-25 C lyophilized;
4 C when reconstituted
Expression System E. Coli
Assay Method(s) SDS-PAGE (purity),
ELISA (activity)
Application Suitable for use in lateral flow, ELISA,
Western Blot.
Each laboratory should determine optimum
working titre for use in it’s particular application
Expiry Date As indicated
Production Date As indicated
Assay Date As indicated
Lot # As indicated